#6499 The CIRCRNA–MIRNA–MRNA REGULATORY NETWORK AND THE POTENTIAL ASSOCIATIONS WITH THE PATHOGENESIS OF SYSTEMIC LUPUS ERYTHEMATOSUS

Abstract Background and Aims This study aimed to explore the possible role of plasma PBMCs circular RNA (circRNA) in systemic lupus erythematosus (SLE). Method Total was extracted from blood samples obtained 10 patients with SLE healthy controls subjected microarray analysis define profile circRNA expression. The quantitative reverse transcription-polymerase chain reaction amplification (qRT-PCR) conducted. overlapped between performed, interactions microRNAs were predicted, miRNA target mRNA GEO database used. Gene ontology pathway performed. Results 131 upregulated 314 significantly downregulated circRNAs identified by Fold change criteria (≥2.0) P < 0.05. qRT-PCR results showed that expression has-circRNA-102531, has-circRNA-103984, has-circRNA-104262 increased SLE, has-circRNA-102972, has-circRNA-102006, has-circRNA-104313 decreased SLE. 28 119 plasma, ubiquitination enriched. Furthermore, circRNA-miRNA-mRNA network constructed after analyzing dataset GSE61635 GEO. comprises 54 circRNAs, 41 miRNAs, 580 mRNAs. In addition, TNF signaling MAPK enriched target. Conclusion We described differentially expressed PBMCs, constructed. network's could be a potential diagnostic biomarker potentially play an important pathogenesis development